Procedures

WWARN is continually updating a library of standardised procedures that offer guidance in the execution of various activities in the fields of clinical, in vitro, pharmacology, data collection and molecular analysis for antimalarial drug resistance researchers. Please read our tools and resources usage guidance.

For resources that relate to other infectious and emerging diseases, visit iddo.org.

Manual size estimation of msp1, msp2 and glurp PCR products on gel

Publication Code: EXT08

Manually determining the size of Plasmodium falciparum polymorphic markers to distinguish recrudesent from reinfecting infections in clinical trials

Version: 1.0

Author(s): Mekong Molecular Surveillance Network

msp1 PCR

Publication Code: EXT05

PCR amplification of the polymorphic marker msp1 to distinguish recrudescences from reinfections

Version: 1.0

Author(s): Mekong Molecular Surveillance Network

Preparing malaria blood smears

Publication Code: CL10

Preparing thick and thin blood smears with Giemsa staining

Version: 2.0

Author(s): WWARN

Copy number estimation of the Plasmodium falciparum pfmdr1 gene

Publication Code: MOL05

Estimating pfmdr1 gene copy number by real-time PCR

Version: v1.1

Culture of Plasmodium falciparum blood stages

Publication Code: INV01

Methods for short- and long term culture of Plasmodium falciparum laboratory strains and fresh isolates

Version: v1.0

Author(s): WWARN

Estimation of Plasmodium falciparum drug susceptibility by the 3H-hypoxanthine uptake inhibition assay

Publication Code: INV07

Drug susceptibility assay measuring radioactive hypoxanthine uptake in laboratory strains and fresh isolates.

Version: v1.0

Author(s): WWARN

Synchronisation of Plasmodium falciparum

Publication Code: INV04

Synchronising culture adapted Plasmodium falciparum isolates in ring stage

Version: v1.1

Author(s): WWARN

DNA extraction by Chelex

Publication Code: EXT02

Extracting Plasmodium spp. DNA from dried blood spots by Chelex 100

Version: 1.0

Author(s): Mekong Molecular Surveillance Network

Estimation of Plasmodium falciparum drug susceptibility ex vivo by HRP2 ELISA

Publication Code: INV09

Drug susceptibility assay measuring histidine-rich protein production by ELISA in fresh isolates

Version: v1.0

Author(s): WWARN

Preparation of drug plates for susceptibility assays

Publication Code: INV03

Preparation of drug dilutions in dry or fresh plates for drug susceptibility assays

Version: v1.2

Author(s): WWARN