Procedures

WWARN is continually updating a library of standardised procedures that offer guidance in the execution of various activities in the fields of clinical, in vitro, pharmacology, data collection and molecular analysis for antimalarial drug resistance researchers. Please read our tools and resources usage guidance.

For resources that relate to other infectious and emerging diseases, visit iddo.org.

Site handling of monitoring visits

Publication Code: QA04

A process for sites to use to help staff involved with attending or arranging monitoring visits

Version: 2.0

WWARN Piperaquine PK PD Study Group NONMEM code

Publication Code: PHA09

NONMEM code for Piperaquine PK/PD Study Group

Version: 1

Ring-stage Survival Assays (RSA) to Evaluate the In-Vitro and Ex-Vivo Susceptibility of Plasmodium Falciparum to Artemisinins

Publication Code: INV10

Evaluting Plasmodium falciparum susceptibility to artemisinins in laboratory strains and fresh isolates

Version: v1.2

DNA Extraction by QIAamp DNA Mini Kit

Publication Code: EXT03

Extracting Plasmodium spp. DNA from dried blood spots by QIAamp DNA Mini Kit

Version: 1.0

Microscopy for the detection, identification and quantification of malaria parasites on stained thick and thin blood films in research settings

Publication Code: EXT10

This manual was developed to guide a move towards common standards for undertaking and reporting research microscopy for malaria parasite detection, identification and quantification.

Version: 1.0

Agarose Gel Electrophoresis of msp1, msp2 and glurp

Publication Code: EXT07

Separating and visualising amplified PCR products of msp1, msp2 and glurp

Version: 1.0

Guidelines for the Cryopreservation of field isolates

Publication Code: EXT11

The purpose of this document is to standardize the methodology for cryopreservation of malaria parasites using glycerolyte

Version: 1.0

PCR and sequencing for genotyping of candidate Plasmodium falciparum artemisinin resistance SNPs in the Kelch 13 gene

Publication Code: MOL08

Genotyping of SNPs in the Kelch 13 gene (PF3D7_1343700), associated with artemisinin resistance

Version: 1.0

Safety assessment and reporting

Publication Code: SAF01

A process for the identification, management, assessment, and reporting of adverse events/adverse drug reactions

Version: v1.0

Manual size estimation of msp1, msp2 and glurp PCR products on gel

Publication Code: EXT08

Manually determining the size of Plasmodium falciparum polymorphic markers to distinguish recrudesent from reinfecting infections in clinical trials

Version: 1.0